![]() CCD cameras also offer convenience by providing a digital record of experiments for data analysis, sharing, and archiving, and by eliminating the need to continually purchase consumables for film development. ![]() Bio-Rad's Clarity Western ECL Substrate is compatible with any HRP-conjugate secondary detection reagent and ideal for both digital and film-based imaging.ĬCD cameras have a linear response over a broad dynamic range - 2–5 orders of magnitude - depending on the bit depth of the system. Additionally, quantitation of data collected by exposure to film requires digitization (that is, scanning of X-ray film with a densitometer). A process termed preflashing can improve linearity, but this requires extra equipment and effort. To gather information from a blot that has both intense and weak signals, multiple exposures are required to produce data for all samples in the linear range of the film. Film is a sensitive medium for capturing the chemiluminescent signal but suffers from a sigmoidal response to light with a narrow region of linear response, which limits its dynamic range. Traditionally, the chemiluminescent signal from blots was detected by X-ray film. Decreasing costs, superior quantitative data quality, and better ease of use are making CCD cameras and imagers preferred over film for these detection techniquesĬomparison of western blot documentation and analysis methods.įor chemiluminescence detection, CCD imaging is the easiest, most accurate, and fastest method. This method also requires the investment in and maintenance of a film developer and often requires processing multiple film sheets to obtain a usable image. X-ray film - widely used for imaging autoradiographic and chemiluminescent blots but suffers from a limited dynamic range as well as a nonlinear response through this range.These screens have a large dynamic range and offer excellent sensitivity and quantitative accuracy with imaging times that are a fraction of those for film Phosphor imagers - laser-based systems capable of imaging storage phosphor screens, which form a latent image when exposed to gamma and beta radiation.Laser scanners allow selective detection of multiple fluorescence labels on gels and blots These imagers can be configured with lasers of different wavelengths, allowing single- or multiplex fluorescence detection. Laser-based imagers - offer the highest sensitivity, resolution, and linear dynamic range and are powerful image acquisition tools for gels and blots with proteins fluorescently labeled or stained with fluorescent dyes such as SYPRO Ruby or Flamingo.Super-cooled CCD cameras reduce image noise, allowing detection of faint luminescent signals with high sensitivity CCD cameras can also be used to detect chemiluminescent signals for which illumination is not needed. Different illumination wavelengths are available for multiplex fluorescence immunodetection. CCD (charge-couple device) cameras - versatile systems that can image gels and blots they can operate with either transillumination provided by light boxes (visible or UV) positioned underneath the gel for imaging a variety of stains (Coomassie, silver, fluorescence) or epi-illumination of blots detected using colorimetric or fluorescence techniques.Densitometers - based on high-performance document scanners with minor modifications (leak-resistant scanning surface, built-in calibration tool) they utilize visible light for the analysis of electrophoresis gels (transmission mode) and blots (reflective mode) stained with visible dyes.Automated features of this imaging software include Auto Spot for finding and calibration as well as Auto Peak for finding and integration.Several instruments and devices are employed to document western blotting results. This software can also produce outputs in histograms. The Bio-Rad GS-670 Imaging Densitometer Software is fitted with a brightness and contrast palette as well as a noise reduction capability. The Bio-Rad GS-670 imaging densitometer can scan and quantify different analyses, including: Transmittance and reflectance modes allow for the virtual scanning of electrophoretic samples, DNAs, RNAs and proteins with this instrument. Options for the actual area of detailed scans are shown afterwards. Scans can be conveniently previewed in a few seconds. ![]() Each unit boasts of high resolution and a bundled software. The Bio-Rad GS-670 is a reliable and cost-effective imaging densitometer, ideal for autoradiographs and western blots. Available software in 1D or 2D but no 3D.General Purpose Interface Bus (GPIB) to either Mac or PC.Equipped with internal interference filters. ![]() ![]() Fast image acquisition (in not more than 15 minutes). ![]()
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